Tyrosinase Inhibitor Screening Kit (Colorimetric)
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Catalog number
K575-100
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Price
Please ask
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Size
100 assays
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Description
Simplest High-Throughput Assay To Screen Tyrosinase Inhibitors
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Summary
• Detection method- Absorbance (OD 510 nm) • Applications- Screening/studying/characterizing tyrosinase inhibitors
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Detection Method
Absorbance (OD 510 nm)
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Applications
Screening/studying/characterizing tyrosinase inhibitors
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Features Benefits
• Rapid, simple & sensitive • Convenient & HTS • Includes inhibitor control Kojic acid
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of Kojic Acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. The assay is also adaptable to a 384-well format.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Short name
Tyrosinase Inhibitor Screening Kit (Colorimetric)
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Technique
colorimetric, colorimetry
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Alternative name
Tyrosinase suppressor detection reagent (colorimetry)
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Alternative technique
assays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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