DNA Polymerase I, Large (Klenow) Fragment

Synonyms

DNA Polymerase I,DNA Polymerase I, Large Fragment,Klenow Fragment,DNA Polymerase I, Klenow,DNA Polymerase I, (Klenow) Fragment,DNA Polymerase I, Large (Klenow) Fragment,2.7.7.7
Alternative_names

DNA Polymerase I,DNA Polymerase I, Large Fragment,Klenow Fragment,DNA Polymerase I, Klenow,DNA Polymerase I, (Klenow) Fragment,DNA Polymerase I, Large (Klenow) Fragment,2.7.7.7
Description

A proteolytic product of E. coli DNA Polymerase I
Recombinant

YES
Source

E. coli
Activity Specifications test method

≥ 20,000 U/mg
Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Molecular Weight

67.9 kDa
Storage Temp

-20ºC
Shipping

Dry Ice
Shelf Life

12 months
Appearance

Liquid protein
Physical form description

Liquid
Background Information

The Large, Klenow Fragment is a proteolytic product of E. coli DNA Polymerase I, which retains 5’→ 3’ polymerase activity and 3’→ 5’ exonuclease activity. It is useful for generating blunt ends by removing the 3’ overhangs and filling the 5’ overhangs. It can be also used to generate probes and second strand cDNA.
Amino acid sequence

Klenow Fragment (amino acids 324 - 928)
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet

Gene target

DNA Polymerase Klenow
Gene symbol

PRKDC, HLA-DOA
Short name

DNA Polymerase I, (Klenow)
Technique

dna
Isotype

Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
Alternative name

Desoxyribonucleic acid polymerizing enzyme I, large (Klenow) Fragment