Caspase-3 Rabbit monoclonal antibody
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Catalog number
BS9865M-100ul
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Price
Please ask
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Size
100ul
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Alternative Name
Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
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Immunogen
Recombinant antibody.
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Swiss Prot
P42574
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Reactivity
Human, Rat
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Applications
WB ICC/IF IHC FC
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Application_all
WB: 1:1000-1:5000 ICC/IF: 1:50-1:100 IHC: 1:50-1:200 FC:1:20-1:50
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Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
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Purification Purity
Protein A affinity purified
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Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Specificity
This antibody detects endogenous levels of Caspase-3 and does not cross-react with related proteins.
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Usage
Western blot (WB) analysis of Caspase-3 Rabbit mAb at 1:1000 dilution Lane1:Hela whole cell lysate Lane2:Jurkat whole cell lysate Lane3:293 whole cell lysate
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BiowMW
~ 35 kDa
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Note
For research use only, not for use in diagnostic procedure.
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Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Caspase-3 Rabbit monoclonal antibody
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Technique
Antibody, Rabbit, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Host
Rabbit, Rabbits
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Alternative name
caspase-3 production species: rabbit monoclonal (antibody to-)
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Alternative technique
antibodies, rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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