Human CellExp™ Carbonic Anhydrase 10/CA10, human recombinant
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Catalog number7485-10
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PricePlease ask
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Size10 ug
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SynonymsCA10, Carbonic Anhydrase X, CARPX, hucep-15
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Alternative_namesCA10, Carbonic Anhydrase X, CARPX, hucep-15
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DescriptionCatalyzes the reversible hydration of carbon dioxide in various biological processes.
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 ug rh CA10 / CARPX enzyme at 100 ng/µl in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
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ResultsMeasured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 ug rh CA10 / CARPX enzyme at 100 ng/µl in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
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Molecular WeightThis protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationCarbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCA10
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Short nameCellExp™ Carbonic Anhydrase 10/CA10, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ Carbonic Anhydrase 10/CA10, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namecarbonic anhydrase 10
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Synonyms gene name
- carbonic anhydrase X
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Carbonic anhydrases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data