Anti-Mouse Stem Cell Antigen 1/Sca-1

  • Catalog number
    103-M477
  • Price
    Please ask
  • Size
    100ug
  • Gene Name
    Stem Cell Antigen 1/Sca-1
  • Species Reactivity
    Mouse (Mus musculus)
  • Synonyms
    Ly6a; TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1
  • Uniprot
    P05533.1
  • Description
    This is a highly purified antibody with strong binding affinity towards the antigen that it has been risen against.Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in.
  • Storage and handling
    Lyophilized antibodies can be shipped at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Reconstituted lyophilised and antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius. Generally, it is recomended for long term storage (up to one year) 25-50% glycerol or ethylene glycol to be added and the vial must be stored at -20°C.
  • Advisory
    In order to retain the quality and the affinity of the antibodies unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
  • Latin name
    Mus musculus
  • Gene target
    Stem   1/Sca-1  
  • Short name
    Anti-Mouse Stem Antigen 1/Sca-1
  • Technique
    antigen, anti-, Mouse, anti, antibody to, antibodies, antigenes, mouses
  • Host
    Rat (Rattus norvegicus)
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Stem Cell Antigen 1/Sca-1 Antibody
  • Alternative technique
    antigenes, murine, antibodies
  • Tissue
    cell, stem
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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