CD3G, human recombinant

• Catalog number
  7313-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, T3G
• Alternative_names
  CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, T3G
• Description
  Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 microgram of protein
• Molecular Weight
  13.1 kDa (117 aa, 23-116 aa + His tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea and 10% glycerol.
• Background Information
  The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. Recombinant human CD3G protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSQSIKGNH LVKVYDYQED GSVLLTCDAE AKNITWFKDG KMIGFLTEDK KKWNLGSNAK DPRGMYQCKG SQNKSKPLQV YYRMCQNCIE LNAATIS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD3G  
• Gene symbol
  CD3G
• Short name
  CD3G, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  CD3g molecule, gamma (CD3-TCR complex), H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA and IMD17 and T3G, CD3G and IDBG-73647 and ENSG00000160654 and 917, protein heterodimerization activity, Plasma membranes, Cd3g and IDBG-158962 and ENSMUSG00000002033 and 12502, CD3G and IDBG-631280 and ENSBTAG00000006453 and 281055

Gene info

• Identity
  HGNC:1675
• Gene
  CD3G
• Long gene name
  CD3g molecule
• Synonyms gene name
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, gamma (CD3-TCR complex)
• GenBank acession
  • X60491
• Locus
  11q23.3
• Discovery year
  1988-05-11
• Entrez gene record
  917
• RefSeq identity
  • NM_000073
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000166971
• Locus Specific Databases
  • CD3Gbase: Mutation registry for autosomal recessiveCD3gamma immunodeficiency
  • LRG_39

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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