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Category
Primary Antibodies
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Long description
Recognizes a protein of 56kDa, which is identified as Lck. p56lck is a membrane-associated non-receptor protein tyrosine kinase that belongs to the Src-family of kinases. The 3A5 MAb shows no cross-reaction with other members of the Src protein kinase family. The unique N-terminal domain of p56lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. Activated p56lck kinase can increase responsiveness of some T cell hybridomas to antigen. The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase. p56lck plays a critical role in T cell development and activation. In humans, p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a recombinant fusion protein containing amino acids 1-213 of human VHL and mouse myeloma p3-NS-1/Ag4-1 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
3A5
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Antigen antibody binding interaction
Mouse anti Lck (p56 lck) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA, Western Blot, Immunoprecipitation
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Antibody s suited for
This antibody can be used for immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) and immunohistochemistry on frozen and formalin/paraffin embedded tissues (2-4 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Kibel A; et al. Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C. Science, 1995, 269:1444-6._x000B__x000B_2. Corless CL; et al. Immunostaining of the von Hippel-Lindau gene product in normal and neoplastic human tissues. Human Pathology, 1997, 28(4):459-64._x000B__x000B_3. Los M; et al. Expression pattern of the von Hippel-Lindau protein in human tissues. Lab Investigation, 1996, 75(2):231-8._x000B__x000B_4. Iliopoulos O; et al. Tumour suppression by the human von Hippel-Lindau gene product. Nature Medicine, 1995, 1(8):822-6.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
