CometChip Kit

Keep at

Mixed
Reagent list
Assay performance

CometAssay, DNA Damage, PCR and Molecular Biology, Oxidative Damage, PARP research.
More advice

For the comet assay procedure GENTAUR also supplies the Comet microscope glass slides. Cellular repair resolves the DNA damage and nucleoids containing specific DNA and the breaks are tested with the comet assay. Performed on Drosophila melanogaster cells or for human DNA damage studies for cancer susceptibility by environmental factors, food habits, sport. A fluorescence microscopy is needed to observe the comet tail where the intensity, length and brightness of the tail indicates the number of DNA lesions and DNA unwinding at neutral pH 7.5. At pH 12.3 the comet assay detects single and double strand breaks, incomplete excision repair sites and cross linking. The DNA amount from the cell nucleus is an indicator of the incurred DNA damage. High throughput is achieved with the 96-Well CometChip the fastest system for high-throughput measurement of DNA damage. Alkali labile sites (ALS) are susceptible of DNA breaching, Miyamae, 1997 and will fast migrate out of the cell from DNA damage, Fairbairn, 1995. The Comet Assay can be applied to both eukaryotic chromosomes and prokaryotic cells. For oncology testing of invasive cancer in the human body the Comet is used by B Vilhar
Other trevi products

Gene target

CometChip Kit
Short name

CometChip Kit
Alternative name

CometChip reagent
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Cell Migration Assays
Concept

Scope note: Specific assays that measure the migration of cells. They are commonly used to measure the migration of immune cells in response to stimuli and the inhibition of immune cell migration by immunosuppressive factors.
Tree numbers
- E01.370.225.500.335
- E01.370.225.812.125
- E05.200.500.335
- E05.200.812.125
- E05.242.335
- E05.478.594.122
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data