Anti-UCHL1 Antibody (7B8-G5-B8)

• Catalog number
  A1332-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Target Alternative Name
  UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Antibody Type
  Monoclonal
• Clone
  7B8-G5-B8
• Species Reactivities
  Human, Monkey
• Immunogen Sequence
  Recombinant human UCHL1/PGP9.5 protein fragments expressed in E.coli
• Accession
  P09936
• Gene Id
  7345
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Purification
  Affinity purified
• Application
  WB, IF
• Positive Control
  WB: U251, IMR32, Raji and COS7 cell lysates; IF: COS7 cells
• Application And Usages
  WB; 1:1000, IF; 1:300
• Description
  Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  UCHL1   7B8-G5-B8  
• Gene symbol
  UCHL1-DT, NDUFA2, RNY4P1, UCHL1, GPANK1
• Short name
  Anti-UCHL1 Antibody (7B8-G5-B8)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG2b
• Alternative name
  Antibody toUCHL1 (antibody to-) (7B8-G5-B8)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:40600
• Gene
  UCHL1-DT
• Long gene name
  UCHL1 divergent transcript
• Synonyms gene
  • UCHL1-AS1
• Synonyms gene name
  • UCHL1 antisense RNA 1 (head to head)
• GenBank acession
  • DB169394
  • CN277289
• Locus
  4p13
• Discovery year
  2012-12-01
• Entrez gene record
  101410542
• RefSeq identity
  • NR_102709
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000160405

Gene info

• Identity
  HGNC:7685
• Gene
  NDUFA2
• Long gene name
  NADH:ubiquinone oxidoreductase subunit A2
• Synonyms gene name
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2 (8kD, B8)
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
• Synonyms
  • B8
• Synonyms name
  • complex I B8 subunit
• GenBank acession
  • AF047185
• Locus
  5q31.3
• Discovery year
  1996-08-30
• Entrez gene record
  4695
• Pubmed identfication
  • 9425316
  • 9763676
• RefSeq identity
  • NM_002488
• Classification
  • NADH:ubiquinone oxidoreductase supernumerary subunits
• VEGA ID
  OTTHUMG00000129505

Gene info

• Identity
  HGNC:10245
• Gene
  RNY4P1
• Long gene name
  RNY4 pseudogene 1
• Synonyms gene name
  • RNA, Y4 small cytoplasmic (associated with Ro protein) pseudogene 1
  • RNA, Ro-associated Y4 pseudogene 1
• Synonyms
  • hY4.B8
• GenBank acession
  • M77131
• Locus
  9q21.13
• Discovery year
  1993-08-25
• Entrez gene record
  6087
• Pubmed identfication
  • 1378808
• RefSeq identity
  • NG_005036

Gene info

• Identity
  HGNC:12513
• Gene
  UCHL1
• Long gene name
  ubiquitin C-terminal hydrolase L1
• Synonyms gene
  • PARK5
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Synonyms
  • PGP9.5
  • Uch-L1
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • BC000332
• Locus
  4p13
• Discovery year
  1991-07-15
• Entrez gene record
  7345
• Pubmed identfication
  • 1840236
• RefSeq identity
  • NM_004181
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000099377

Gene info

• Identity
  HGNC:13920
• Gene
  GPANK1
• Long gene name
  G-patch domain and ankyrin repeats 1
• Synonyms gene
  • BAT4
• Synonyms gene name
  • HLA-B associated transcript 4
• Synonyms
  • G5
  • D6S54E
  • GPATCH10
  • ANKRD59
• Synonyms name
  • G patch domain containing 10
  • ankyrin repeat domain 59
• Locus
  6p21.33
• Discovery year
  2001-07-13
• Entrez gene record
  7918
• Pubmed identfication
  • 2911734
  • 2813433
• RefSeq identity
  • NM_033177
• Classification
  • G-patch domain containing
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000031174

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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