Neuropoietin, murine recombinant

• Catalog number
  7180-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  NPO, NP
• Alternative_names
  NPO, NP
• Description
  Promotes the survival of embryonic motor neurons
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by the dose dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is 0.5-0.8 ug/ml.
• Biological activity
  Determined by the dose dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is 0.5-0.8 ug/ml.
• Molecular Weight
  19.8 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 2.5 mM Tris, pH 10.2 and 0.5 mM DTT
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-α component, gp130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues.
• Amino acid sequence
  MAPISPSEPI GQAYSLALYM QKNTSALLQT YLQHQGSPFS DPGFSAPELQ LSTLPSAAVS FKTWHAMEDA ERLSRAQGAF LALTQHLQLV GDDQSYLNPG SPILLAQLGA ARLRAQGLLG NMAAIMTALG LPIPPEEDTL GFVPFGASAF ERKCRGYIVT REYGHWTDRA VRDLALLKAK YSA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Neuropoietin   murine  
• Short name
  Neuropoietin, murine recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Neuropoietin, murine Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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