K11-linked Tetra-Ubiquitin

Synonyms

K11-linked Tetra-Ubiquitin
Description

Substrate for defined length polyubiquitination
Purity by SDS PAGE

≥95%
Assay

Western Blot
Molecular Weight

34.205 kDa (Band migrates faster on gels)
Storage Temp

-80°C
Shipping

Dry ice
Shelf Life

12 months
Concentration

2.5 mg/ml
Appearance

Liquid
Physical form description

2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.
Background Information

Increasingly, researchers are focusing on the role poly-ubiquitin chains linked through K11. This post-translational modification has been linked to the ERAD cycle as a signal, similar to K48 linkage, for proteasomal degradation. More recently, K11 linkage appears to play an important role in cell cycle signaling, as it is associated with the anaphase promoting complex (APC) of ubiquitination machinery. These tetra-ubiquitin chains are generated from the enzymatic linkage (UBE2S) of wild-type ubiquitin through lysine 11 and purified to >95% homogeneity by ion exchange chromatography.
Handling

Centrifuge the vial prior to opening.
Usage

For Research Use Only! Not to be used in humans
Datasheet