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Donkey Anti Human IgG Antibody H

Donkey Anti Human IgG Antibody H is available 3 times from Bioss secondary antibodies labs

bs-0297D | Donkey Anti-Human IgG Antibody (H+L) size: 1mg | 158.02 USD

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bs-0297D-Biotin | Donkey Anti-Human IgG Antibody (H+L), Biotin Conjugated size: 0.2ml | 135.60 USD

Category Conjugated Secondary Antibody
Conjugation Biotin
Concentration 1ug/ul
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test Bioss Secondary Antibodies supplies antibodies that are for research of human proteins.
Additional conjugation Biotinylated
Latin name Equus africanus asinus
About Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotype IgG
French translation anticorps
Gene targetH+L Conjugated
Short name Anti- IgG Antibody (H+L) Conjugated
Label Biotin
Alternative name Donkey antibody to-H. sapiens Immunoglobulin G (Antibody to) (heavy and light chain), biotinilated coupled
MeSH Data
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Human#.jpg
Product files

bs-0297D-HRP | Donkey Anti-Human IgG Antibody (H+L), HRP Conjugated size: 0.2ml | 135.60 USD

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Host Organism Donkey
Target antigen Human IgG
Modification Unmodified
Modification site None
Clonality Polyclonal antibody
Clone Polyclonal antibody
Tested applications WB, ELISA, IHC-P, IHC-F
Recommended dilutions WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
Cross reactivity Human
Background of the target antigen Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Purification method This antibody has been purified via Protein A.
Storage conditions Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
Technique anti-Human, Antibody, anti, IgG
Host Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
Isotype IgG
Species Human
Alternative technique antibodies
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Donkey Anti-Human IgG Antibody (H+L), Biotin Conjugated
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