Caspase-1, human recombinant

• Catalog number
  1081-100
• Price
  Please ask
• Size
  100 units

• Synonyms
  Caspase-1
• Alternative_names
  Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, IL-1 beta-converting enzyme, p45
• Description
  A cysteine protease involved in apoptosis
• Recombinant
  Yes
• Source
  E. coli.
• Assay
  SDS-PAGE
• Activity Specifications test method
  > 5000 units/mg
• Unit Definition
  One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Molecular Weight
  large (20 kD) and small (10 kD) subunitsk
• Storage Temp
  -70°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Semi-Dry powder
• Physical form description
  Lyophilized powder
• Reconstitution Instructions
  Reconstitute to 1 unit per µl in water.
• Background Information
  Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Caspase-1  
• Gene symbol
  CASP12, CASP1P3, CASP1
• Short name
  Caspase-1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  caspase-1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:19004
• Gene
  CASP12
• Long gene name
  caspase 12 (gene/pseudogene)
• Synonyms gene
  • CASP12P1
• Synonyms gene name
  • caspase 12 pseudogene 1
  • caspase 12
• GenBank acession
  • AF464191
• Locus
  11q22.3
• Discovery year
  2002-07-25
• Entrez gene record
  100506742
• Pubmed identfication
  • 12054529
  • 9038361
  • 16917906
  • 16532395
• RefSeq identity
  • NM_001191016
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000154965

Gene info

• Identity
  HGNC:43777
• Gene
  CASP1P3
• Long gene name
  caspase 1 pseudogene 3
• Synonyms gene name
  • caspase 1, apoptosis-related cysteine peptidase pseudogene 3
• Locus
  11q22.3
• Discovery year
  2012-02-24
• Entrez gene record
  100422634
• RefSeq identity
  • NG_024409

Gene info

• Identity
  HGNC:1499
• Gene
  CASP1
• Long gene name
  caspase 1
• Synonyms gene
  • IL1BC
• Synonyms gene name
  • caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase)
  • caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
  • caspase 1, apoptosis-related cysteine peptidase
• Synonyms
  • ICE
• Synonyms name
  • caspase-1
  • interleukin 1, beta, convertase
• GenBank acession
  • U13697
• Locus
  11q22.3
• Discovery year
  1992-05-07
• Entrez gene record
  834
• Pubmed identfication
  • 1373520
  • 9250871
• RefSeq identity
  • NM_033292
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000048072

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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