HAMSTER ANTI MOUSE CD11c:FITC Antibody
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Catalog number
GEN210673
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Price
Please ask
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Size
500ug
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Also known as
CD11c
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Other names
integrin alpha-X; Integrin alpha-X; integrin alpha-X; integrin aX; complement receptor 4; CD11C (p150) alpha polypeptide; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; leukocyte adhesion glycoprotein p150,95 alpha chain; integrin alpha X; CD11 antigen-like family member C; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD11c
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Gene name synonims
N/A
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Other gene names
Itgax; Itgax; Cr4; N418; Cd11c; AI449405; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG
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Clone
N418
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Host organism
Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD11c This item recognises the murine homologue of human CD11c, which is a 150/90kD member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 0.5 mg/ml
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Flow Cytometry (FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
ITGAX
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Short name
ANTI MOUSE CD11c:FITC Antibody
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Technique
anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
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Host
Hamster
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Label
FITC
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Species
Mouse, Hamsters, Mouses
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Alternative name
Anti-HAMSTER ANTI MOUSE CD11c:FITC
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Alternative technique
antibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
integrin subunit alpha X
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Synonyms gene
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Synonyms gene name
- integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
- integrin, alpha X (complement component 3 receptor 4 subunit)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-08-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Integrin alpha subunits
- Complement system regulators and receptors
- CD molecules
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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