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Host Source
Rabbit
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Clone Name
Polyclonal
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Product Category
Veterinary
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Species Reactivity
Mouse
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
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Immunogen
Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with FITC, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days
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Datasheet link
NA
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with FITC, Secondary Antibodies
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Technique
anti mouse, Rabbit, Mouse, anti, FITC, FC, antibody to, anti mouse antibodies, antibody Conjugates, Fluorescein, mouses
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Host
Rabbit, Rabbits
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Isotype
NA, IgG1, IgG2a, IgG2b, IgG3
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
production species: rabbit antibody to Mouse IgG1 IgG2a IgG2b IgG3 (fragment c specific), coupled including fluorecein, secondary detector Antibody Antibodies
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Alternative technique
rabbit-anti, murine, antibodies, fluorescine
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