T4 DNA Ligase - 2000 units/µl - 400,000 units

  • Catalog number
    3217
  • Price
    Please ask
  • Size
    400000 units
  • Description
    Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Intact Genomics T4 DNA Ligase displays significantly higher ligation efficiency than the nearest competitor. Product Includes: T4 DNA Ligase 10x T4 DNA Ligase Reaction Buffer
  • Shipping Condition
    Ships with Dry Ice
  • Gene target
    DNA   Ligase   2000   units/µl   400   000   units  
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    T4 DNA Ligase - 2000 units/µl - 400 000 units
  • Technique
    dna
  • Alternative name
    T4 Desoxyribonucleic acid Ligase - 2000 units/µl - 400,000 units
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
  • Tree numbers
    • E05.393.620.311
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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