Cell Meter FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

  • Catalog number
    22839
  • Price
    Please ask
  • Size
    100 Tests
  • Description
    Cell Meter FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* is used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS).
  • Shipping
    Room Temperature
  • Storage
    Freezer
  • Shelflife
    6 months
  • Excitation Wavelength
    490 nm
  • Emission Wavelength
    520 nm
  • Personal precautions
    Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
  • Uses
    Laboratory chemicals *For Research Use Only*
  • Ordering
    To order Cell Meter FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*, please use the Cat. Nr.22839 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*
  • Additional description
    This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. aat has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies. For cells, cell lines and tissues in culture till half confluency. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
  • Test
    aat supplies other types of Assays as 1. Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
  • Properties
    This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
    Meter   Annexin   Binding   Kit   Optimized   for  
  • Short name
    Meter FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for *
  • Technique
    Flow Cytometry, apoptosis, Assay, FITC, Flow, apoptosis pathway, assays, Fluorescein
  • Host
    Assay
  • Label
    FITC
  • Alternative name
    cellular Meter fluorecein-Annexin V Binding programmed cellular destruction test reagent *Optimized to measure Flow Cytometry*
  • Alternative technique
    celldeath, arrays, fluorescine, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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