Human Alpha-2-Macroglobulin (a2M) CLIA Kit, 96 well plate
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Catalog numberEKU08930
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PricePlease ask
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Size5x96T
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Verified reactivityHomo sapiens (Human)
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Detection limits0.39-100 ng/mL
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Sensitivity limit0,05 ng/mL
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Samples to be analyzedSerum, plasma, urine and other biological fluids
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Assay duration2 hours, 40min
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Verified applicationsCLIA
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Assay classSandwich
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ELISA detectionChemiluminescent
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Protein numberP01023
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Alternate protein numberPlease refer to SwissProt
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Gene number2
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Gene nameAlpha-2-Macroglobulin
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Alternate gene nameA2MG; A2mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-2-Macroglobulin (a2M) level in the sample or standard.;
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaTumor immunity;Infection immunity;Hematology;
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WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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DescriptionThe Alpha-2-Macroglobulin (a2M) CLIA Kit, 96 is a α- or alpha protein sometimes glycoprotein present in blood. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolMIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2, KRTAP13-2, SNORD114-2, SNORD116-2
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Short nameAlpha-2-Macroglobulin (a2M) CLIA Kit, 96
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SpeciesHuman, Humans
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Alternative nameH. sapiens a-2-Macroglobulin (alpha-2-macroglobulin) CLIA reagent, 96 well plate
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Alternative techniquekits
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Alternative to gene targetalpha-2-macroglobulin, A2MD and CPAMD5 and FWP007 and S863-7, A2M and IDBG-17623 and ENSG00000175899 and 2, calcium-dependent protein binding, Extracellular, A2m and IDBG-184734 and ENSMUSG00000030111 and 232345, A2M and IDBG-639675 and ENSBTAG00000018137 and 513856
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Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 521-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR515 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 509-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR506 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR7 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 329-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2005-07-11
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR329 family
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 2
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namekeratin associated protein 13-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-21
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Entrez gene record
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Pubmed identfication
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Classification
- Keratin associated proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-2
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box