Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1, Clone S398A-34

• Catalog number
  SMC-489D-A565
• Price
  Please ask
• Size
  100 µg

• Clone
  S398A-34
• Immunogen
  Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
• Antibody s full description
  Mouse Anti-Rat GABA A Receptor Monoclonal IgG1 Antibody, Clone: S398A-34: ATTO 565
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  GABA A receptor subunit alpha 4 Antibody, GABRA4 Antibody, Gamma aminobutyric acid A receptor Alpha 4 Antibody
• Raised in
  Mouse
• Antibody s target
  GABA A Receptor
• Primary research fields
  Neuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). GABA-A Receptor Alpha 4 is associated with epilepsy (3), and research suggests that alterations in GABA- A Receptors subtypes may be involved in the resistance to antiepileptic drugs (4).
• Bibliography
  1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Grabenstatter H.L., et al. (2014) Epilepsia. 55(11): 1826-1833. 4. Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31(2): 169-187.
• Released date
  10-Mar-2015
• PubMed number
  27767042
• Tested applications
  Immunocytochemistry/Immunofluorescence
• Tested reactivity
  Rat
• NCBI number
  NP_542154.3
• Gene number
  140675
• Protein number
  P28471
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34 (SMC-489). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody (SMC-489) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha4 Antibody (D) Composite. Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (SMC-489). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (SMC-489) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa. Mouse Anti-GABA-A Receptor Alpha4 Antibody [S398A-34] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-489) Mouse Anti-GABA-A-Receptor-Alpha4 Antibody [S398A-34] used in Western Blot (WB) on Mouse Brain (SMC-489)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  GABA-A   Receptor   Alpha4   Monoclonal   IgG1   S398A-34  
• Gene symbol
  SNORD115-34, IGKV3D-34, IGKV3-34, IGHV4-34, IGLV2-34, ABAT, IGHV7-34-1, HLA-A, RNU6-34P, IGHV5-10-1
• Short name
  Mouse Anti- GABA-A Receptor Alpha4 Monoclonal IgG1, Clone S398A-34
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG1, IgG1
• Label
  ATTO 565
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat GABA-A Receptor Alpha4 monoclonal IgG1, clonality S398A-34
• Alternative technique
  murine, antibodies
• Clone name
  S398A-34

Gene info

• Identity
  HGNC:33053
• Gene
  SNORD115-34
• Long gene name
  small nucleolar RNA, C/D box 115-34
• Synonyms
  • HBII-52-34
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033808
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003349.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5828
• Gene
  IGKV3D-34
• Long gene name
  immunoglobulin kappa variable 3D-34 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 3D-34
  • immunoglobulin kappa variable 3D-34 pseudogene
• GenBank acession
  • X71895
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28871
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151601

Gene info

• Identity
  HGNC:5820
• Gene
  IGKV3-34
• Long gene name
  immunoglobulin kappa variable 3-34 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 3-34
  • immunoglobulin kappa variable 3-34 pseudogene
• GenBank acession
  • X71891
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28909
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151693

Gene info

• Identity
  HGNC:5650
• Gene
  IGHV4-34
• Long gene name
  immunoglobulin heavy variable 4-34
• GenBank acession
  • X92278
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28395
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152074

Gene info

• Identity
  HGNC:5893
• Gene
  IGLV2-34
• Long gene name
  immunoglobulin lambda variable 2-34 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 2-34
  • immunoglobulin lambda variable 2-34 pseudogene
• GenBank acession
  • D87013
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28810
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151174

Gene info

• Identity
  HGNC:23
• Gene
  ABAT
• Long gene name
  4-aminobutyrate aminotransferase
• Synonyms
  • GABAT
  • GABA-T
• Synonyms name
  • 4-aminobutyrate transaminase
  • gamma-aminobutyrate aminotransferase
  • GABA transaminase
• GenBank acession
  • L32961
• Locus
  16p13.2
• Discovery year
  1996-03-13
• Entrez gene record
  18
• Pubmed identfication
  • 7721088
• RefSeq identity
  • NM_020686
• VEGA ID
  OTTHUMG00000048201

Gene info

• Identity
  HGNC:5664
• Gene
  IGHV7-34-1
• Long gene name
  immunoglobulin heavy variable 7-34-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 7-34-1
  • immunoglobulin heavy variable 7-34-1 pseudogene
• GenBank acession
  • AB019439
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28382
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152085

Gene info

• Identity
  HGNC:4931
• Gene
  HLA-A
• Long gene name
  major histocompatibility complex, class I, A
• GenBank acession
  • D32129
• Locus
  6p22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3105
• Pubmed identfication
  • 8838351
• RefSeq identity
  • NM_002116
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000130501
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:34278
• Gene
  RNU6-34P
• Long gene name
  RNA, U6 small nuclear 34, pseudogene
• Synonyms gene
  • RNU6-34
• Synonyms gene name
  • RNA, U6 small nuclear 34
• Locus
  4q22.3
• Discovery year
  2008-06-10
• Entrez gene record
  100873749
• RefSeq identity
  • NR_046492

Gene info

• Identity
  HGNC:5661
• Gene
  IGHV5-10-1
• Long gene name
  immunoglobulin heavy variable 5-10-1
• Synonyms gene
  • IGHV5-A
• Synonyms gene name
  • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
  • immunoglobulin heavy variable 5-A (provisional)
• Synonyms
  • IGHV5-a
• GenBank acession
  • X92227
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28386
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191143

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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