Mouse anti Human IgG4 (pFc subclass specific), conjugated with FITC, Secondary Antibodies

• Catalog number
  MAHu-IgG4-FITC
• Price
  Please ask
• Size
  0,2mg

• Host Source
  Mouse
• Clone Name
  NI 315 (HP 6206)
• Product Category
  Immunology
• Species Reactivity
  Human
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
• Immunogen
  Highly purified monoclonal IgG4 isolated from human serum.
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  Mouse anti Human IgG4 (pFc subclass specific), conjugated with FITC, Secondary Antibodies philized product is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 C, not refrozen, and preferably used the same day. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days
• Datasheet link
  NA
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  IgG4   pFc   subclass   specific   conjugated   with   Secondary   Antibodies  
• Short name
  Mouse anti IgG4 (pFc subclass specific), conjugated with FITC, Secondary Antibodies
• Technique
  anti human, Mouse, anti, FITC, antibody to, anti human antibodies, antibody Conjugates, Fluorescein, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  FITC
• Species
  Human, Humans, Mouses
• Alternative name
  Mouse antibody to H. sapiens IgG4 (pFc subclass specific), coupled including fluorecein, secondary detector Antibody Antibodies
• Alternative technique
  murine, antibodies, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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