CD31/PECAM-1 (A280) polyclonal antibody

Alternative Name

Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1
Immunogen

Synthetic peptide, corresponding to amino acids 240-290 of Human CD31.
Swiss Prot

P16284
Reactivity

Human,Mouse,Rat
Applications

WB
Application_all

WB: 1:500~1:1000
Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity

CD31/PECAM-1 (A280) polyclonal antibody detects endogenous levels of CD31 protein.
Usage

Western blot (WB) analysis of CD31 (A280) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate
BiowMW

~ 130 kDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

CD31/PECAM-1 A280
Gene symbol

PECAM1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
Short name

CD31/PECAM-1 (A280) polyclonal antibody
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit
Alternative name

CD31/PECAM-1 (A280) polyclonal (antibody to-)
Alternative technique

polyclonals, antibodies

Identity

HGNC:8823
Gene

PECAM1
Long gene name

platelet and endothelial cell adhesion molecule 1
Synonyms gene name
- platelet/endothelial cell adhesion molecule 1
Synonyms
- CD31
Synonyms name
- CD31 antigen
GenBank acession
- L34631
- M37780
Locus

17q23.3
Discovery year

1995-11-29
Entrez gene record

5175
Pubmed identfication
- 8661055
- 1690453
RefSeq identity
- NM_000442
Classification
- CD molecules
- Immunoglobulin like domain containing
- Ig-like cell adhesion molecule family
VEGA ID

OTTHUMG00000175829

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data