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Clone
S68-6
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Immunogen
Fusion protein amino acids 1751-1946 (C-terminus) of human Nav1.7
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Antibody s full description
Mouse Anti-Human Nav1.7 Monoclonal IgG1 Antibody, Clone: S68-6: ATTO 680
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
ETHA Antibody, hNE Na Antibody, NE NA Antibody, PN1 Antibody, SCN9A Antibody, voltage gated sodium channel subunit alpha Nav1 Antibody, peripheral sodium channel 1 Antibody, neuroendocrine sodium channel Antibody
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Raised in
Mouse
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Antibody s target
Nav1.7
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Primary research fields
Neuroscience, Ion Channels, Sodium Channels, Voltage-Gated Sodium Channels, Cancer, Cell Signaling
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Brandname
None
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Antibodies applications
WB, ICC/IF, IP, AM
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Antibody s reactivity
Human, Mouse, Rat, Hamster
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~230kDa. No cross-reactivity against other Nav channels.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-314 was sufficient for detection of Nav1.7 in 10 µg of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane , Synapse
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Nav1.7 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.7 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.7 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (1-3).
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Bibliography
1. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58. 2. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212. 3. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P. (2008) Mol Pain. 4: 21.
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Released date
6-Jul-2009
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PubMed number
25339738
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Tested applications
Immunocytochemistry/Immunofluorescence
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Tested reactivity
Mouse
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NCBI number
NP_002968.1
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Gene number
6335
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Protein number
Q15858
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6 (SMC-314). Tissue: Brain Slice. Species: Mouse. Fixation: Frozen sections. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (SMC-314) at 1:1000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain. Western Blot analysis of hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6 (SMC-314). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (SMC-314) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6 (SMC-314). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (SMC-314) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone S68-6 (SMC-314). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (SMC-314) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-Nav1.7 Sodium Channel Antibody [S68-6] used in Immunohistochemistry (IHC) on Mouse Brain Slice (SMC-314) Mouse Anti-Nav1.7 Sodium Channel Antibody [S68-6] used in Western Blot (WB) on hamster CHO cells (SMC-314) Mouse Anti-Nav1.7 Sodium Channel Antibody [S68-6] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-314) Mouse Anti-Nav1.7 Sodium Channel Antibody [S68-6] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-314)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
