Anti-5-Methylcytosine Antibody

• Catalog number
  A1294-50
• Price
  Please ask
• Size
  50 µg

• Antibody Target
  5-mc
• Target Alternative Name
  5-mc, 5mc
• Antibody Type
  Monoclonal
• Clone
  33D3
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  Modified ribonucleoside, specific for the presence of a methyl group on carbon 5 of the pyrimidine ring
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.4
• Purification
  Affinity purified
• Application
  WB, ELISA, MeDIP/ChIP, IF, , IHC, FC
• Application And Usages
  WB; 1:250, ELISA: 1:10000, MeDIP/ChIP: 1-2 µg per IP, IF: 1:500, IHC Perform heat mediated antigen retrieval before commencing with IHC staining protocol, ICC; 0.5-5 µg/ml, FC; 10µl of working dilution to label 10 x 106 cells in 100µl
• Description
  5-methylcytosine (5-mC) is a modified base present in nucleic acids of plants and vertebrates. DNA methylation is a post-replicative process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Carcinogenesis is associated with alterations of the DNA methylation pattern: a global hypomethylation is often detected in tumor tissues when compared to their normal counterpart. Simultaneously local hypermethylation sites are observed.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  5-Methylcytosine  
• Short name
  Anti-5-Methylcytosine Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG1, Lambda
• Alternative name
  Antibody to5-Methylcytosine (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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