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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser241 of human PDK1 (AA238-244)
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Antibody s target
Human PDK1 (pSer241)
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Antibody s full description
Rabbit Anti-Human PDK1 (pSer241) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
PDK1 Antibody, PDPK1 Antibody, PDPK1_HUMAN Antibody, PDPK2 Antibody, PkB kinase Antibody, PkB kinase like gene 1 Antibody, PkB like 1 Antibody, PRO0461 Antibody, Protein kinase Antibody3 phosphoinositide dependent protein kinase 1 Antibody, 3-phosphoinositide-dependent protein kinase 1 Antibody, hPDK 1 Antibody, hPDK1 Antibody, MGC20087 Antibody, MGC35290 Antibody, OTTHUMP00000159109 Antibody, OTTHUMP00000159110 Antibody, OTTHUMP00000174525 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~63kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1050 was sufficient for detection of PDK1 (pSer241) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Cell Membrane, Cell Junction, Focal Adhesion
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Tissue specificity
Expressed ubiquitously with highest expression in blood vessels and the brain. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_002601
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Gene number
5170
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Protein number
O15530
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PDK1, PDPK1, APC
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Short name
Rabbit Anti- PDK1 (pSer241) Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman PDK1 (pSer241) polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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