Jurkat, Clone E6-1 Cell Line

Abbreviation

Jurkat, Clone E6-1
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Lymphoblast
Growth_properties

Suspension
Doubling_time

NA
Background

Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen(both types of stimulants are needed to induce IL-2 production.
Biosafety

1
Medium

RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

Receptor expression: T cell antigen receptor, expressed
Antigen_expression

CD3; Homo sapiens, expressed
Gene_expression

Interleukin-2(Interleukin 2, Il-2), Cd3; Homo Sapiens, Expressed
Applications

Transfection.
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Gene target

Jurkat E6-1 Line
Gene symbol

UBE3A, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
Short name

Jurkat, Clone E6-1 Line
Species

Homo sapiens, Human
Alternative name

Jurkat, clonality E6-1 cellular Line
Clone name

E6-1
Tissue

Peripheral blood

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081