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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser222 of human SRPK1 (AA219-225)
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Antibody s target
Human SRPK1 (pSer222)
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Antibody s full description
Rabbit Anti-Human SRPK1 (pSer222) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Serine/arginine rich protein specific kinase 1 Antibody, Serine/arginine rich splicing factor kinase 1 Antibody, Serine/arginine-rich protein-specific kinase 1 Antibody, Serine/threonine protein kinase SRPK1 Antibody, Serine/threonine-protein kinase SRPK1 Antibody, SFRS protein kinase 1 Antibody, SFRSK1 Antibody, SR protein kinase 1 Antibody, SR protein specific kinase 1 Antibody, SR-protein-specific kinase 1 Antibody, SRPK1 Antibody, SRPK1_HUMAN Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 74.325 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1084 was sufficient for detection of SRPK1 (pSer222) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Nucleus matrix, Microsome
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Tissue specificity
Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2. Highly expressed in different erythroid and lymphoid cell lines, with isoform 2 being far more abundant than isoform 1.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_003128
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Gene number
6732
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Protein number
Q96SB4
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
SRPK1
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Short name
Rabbit Anti- SRPK1 (pSer222) Polyclonal: ATTO 633
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 633
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman SRSF protein kinase 1 (pSer222) polyclonal: ATTO 633
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
SRSF protein kinase 1, SFRSK1, SRPK1 and IDBG-84414 and ENSG00000096063 and 6732, transferase activity, nuclei, Srpk1 and IDBG-158718 and ENSMUSG00000004865 and 20815, SRPK1 and IDBG-629997 and ENSBTAG00000016838 and 512631
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