Modification Not modified
Clonality Monoclonal Antibodies
Host organism Mouse (Mus musculus)
Background information Hepatocyte specific antigen is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. Hepatcoyte labels an antigen in the mitochondrial fraction of the liver homogenates. Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes. HSA (Hepatocyte Specific Antigen) has been demonstrated consistently in the vast majority of hepatocellular carcinomas. Studies have shown the utility of HSA in the differential diagnosis of hepatocellular carcinoma, cholangiocarcinoma and hepatoblastomas.
Specificity Highly specific antibody against Hepatocyte
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Conjugated with ALEXA FLUOR 594
Gene ID N/A
Cross Reactive species Human (Homo sapiens)
Cross Reactive Species details No significant cross reactivity is observed
Synonyms Hepatocyte specic antigen; Cell growth inhibiting protein 42; Growth inhibiting protein 20; Human ALB; Human serum albumin.
Alternative name 1 Hepatocyte(2H5) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated
Concentration 1ug per 1ul
Purification method Purified by Protein G.
Advisory Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
Type Conjugated Primary Antibody
Excitation emission 590nm/617nm
Storage Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Target Protein/Peptide Hepatocyte
Alternative name 2 Hepatocyte(2H5) Monoclonal anti-, ALEXA FLUOR 594 Conjugated
Antigen Source human liver extract
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.