Rat NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit
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Catalog number
EKE62217
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Price
Please ask
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Size
96 Tests
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Estimated Turnaround Time
6-11 business days
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Target Name
NA
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Target s alterntive name
NADPH
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Species Reactivity
Rat
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Sample Type
Serum, Plasma, Biological Fluids
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Detection Range
31.25-2000 ng/mL
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Sensitivity
18.75 ng/mL
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Precision
NA
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Detection Method
Colorimetric
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Assay Time
2.5 hours
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Application
For research use only. Not for diagnostic procedures.
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Assay Type
Competitive
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Shipping Conditions
Ice packs
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Storage Temperature
Short term: 4°C; Long term: see manual.
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Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf life
Use Rat NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit before 6 months
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Specificity
NA
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Cross Activity
No significant cross-reactivity or interference was observed.
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Test Principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat NADPH. During the reaction, Rat NADPH in the sample or standard competes with a fixed amount of Rat NADPH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat NADPH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat NADPH in the samples is then determined by comparing the OD of the samples to the standard curve.
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Stability
NA
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Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
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Research Area
NA
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
DUOX1, DUOX2
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Short name
NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
Rat, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Rat, Rats
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Alternative name
Rat NADPH (Nicotinamide Adenine Dinucleotide Phosphate) Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
dual oxidase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-05-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
dual oxidase 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
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