Human CellExp™ KLK-7 / Kallikrein-7, human recombinant

• Catalog number
  7510-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  KLK7, Kallikrein-7, PRSS6, SCCE, hK7, hSCCE
• Alternative_names
  KLK7, Kallikrein-7, PRSS6, SCCE, hK7, hSCCE
• Description
  ≥95% pure, active, human cell expressed, human recombinant KLK-7 / Kallikrein-7 - a protein involved in metastasis in several cancers
• Recombinant
  Yes
• Source
  HEK293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) NH2. The specific activity is >130 pmoles / min / ug.
• Results
  >130 pmoles / min / ug
• Molecular Weight
  This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 26.1 kDa. The predicted N-terminus is Glu 23. DTT-reduced Protein migrates as 29-33 kDa in SDS-PAGE due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
• Background Information
  Kallikrein-7 (KLK7) is also known as kallikrein-related peptidase 7, Stratum corneum chymotryptic enzyme, Serine protease 6, KLK7, and PRSS6, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. KLK7 is secreted as an inactive zymogen in the stratum granulosum layer of the epidermis, requiring proteolytic cleavage of the short N-terminal pro-region to liberate activated enzyme. This may be performed by KLK5 or matriptase, which are in vitro activators of KLK7. Once active, KLK7 is able to cleave desmocollin and corneodesmosin. KLK7 activity is regulated by a number of endogenous protein inhibitors including LEKTI, SPINK6, elafin and alpha-2-Macroglobulin-like 1. Both Zn2+ and Cu2+ ions are also able to inhibit KLK7. Dysregulation of KLK7 has been linked to several skin disorders, and overexpression of KLK7 may provide a route for metastasis in several cancers.
• Amino acid sequence
  Glu 23 – Arg 253
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   KLK-7   Kallikrein-7  
• Gene symbol
  KLK14, KLK9, KLK12, KLK7, KLK13, PIRC34, PIRC33, KLK5, PIRC32, SNORD114-7
• Short name
  CellExp™ KLK-7 / Kallikrein-7, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ KLK-7 / Kallikrein-7, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6362
• Gene
  KLK14
• Long gene name
  kallikrein related peptidase 14
• Synonyms gene name
  • kallikrein 14
• Synonyms
  • KLK-L6
• GenBank acession
  • AF283670
• Locus
  19q13.41
• Discovery year
  2000-03-16
• Entrez gene record
  43847
• Pubmed identfication
  • 16800724
  • 16800723
• RefSeq identity
  • NM_022046
• Classification
  • Kallikreins
• VEGA ID
  OTTHUMG00000143721

Gene info

• Identity
  HGNC:6370
• Gene
  KLK9
• Long gene name
  kallikrein related peptidase 9
• Synonyms gene name
  • kallikrein 9
• Synonyms
  • KLK-L3
• GenBank acession
  • AF135026
• Locus
  19q13.41
• Discovery year
  2000-03-16
• Entrez gene record
  284366
• Pubmed identfication
  • 16800724
  • 16800723
• RefSeq identity
  • NM_012315
• Classification
  • Kallikreins
• VEGA ID
  OTTHUMG00000183087

Gene info

• Identity
  HGNC:6360
• Gene
  KLK12
• Long gene name
  kallikrein related peptidase 12
• Synonyms gene name
  • kallikrein 12
• Synonyms
  • KLK-L5
• Locus
  19q13.41
• Discovery year
  2000-03-16
• Entrez gene record
  43849
• Pubmed identfication
  • 16800724
  • 16800723
• RefSeq identity
  • NM_019598
• Classification
  • Kallikreins
• VEGA ID
  OTTHUMG00000165806

Gene info

• Identity
  HGNC:6368
• Gene
  KLK7
• Long gene name
  kallikrein related peptidase 7
• Synonyms gene
  • PRSS6
• Synonyms gene name
  • kallikrein 7 (chymotryptic, stratum corneum)
• Synonyms
  • SCCE
• GenBank acession
  • L33404
• Locus
  19q13.41
• Discovery year
  1995-06-14
• Entrez gene record
  5650
• Pubmed identfication
  • 8034709
  • 16800724
  • 16800723
• RefSeq identity
  • NM_005046
• Classification
  • Serine proteases
  • Kallikreins
• VEGA ID
  OTTHUMG00000182917

Gene info

• Identity
  HGNC:6361
• Gene
  KLK13
• Long gene name
  kallikrein related peptidase 13
• Synonyms gene name
  • kallikrein 13
• Synonyms
  • KLK-L4
• Locus
  19q13.41
• Discovery year
  2000-03-16
• Entrez gene record
  26085
• Pubmed identfication
  • 16800724
  • 16800723
• RefSeq identity
  • NM_015596
• Classification
  • Kallikreins
• VEGA ID
  OTTHUMG00000182912

Gene info

• Identity
  HGNC:37538
• Gene
  PIRC34
• Long gene name
  piwi-interacting RNA cluster 34
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313890
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37537
• Gene
  PIRC33
• Long gene name
  piwi-interacting RNA cluster 33
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313854
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:6366
• Gene
  KLK5
• Long gene name
  kallikrein related peptidase 5
• Synonyms gene name
  • kallikrein 5
• Synonyms
  • SCTE
  • KLK-L2
• GenBank acession
  • AF135028
• Locus
  19q13.41
• Discovery year
  2000-03-16
• Entrez gene record
  25818
• Pubmed identfication
  • 10514489
  • 10608802
  • 16800724
  • 17012259
• RefSeq identity
  • NM_012427
• Classification
  • Kallikreins
• VEGA ID
  OTTHUMG00000183097

Gene info

• Identity
  HGNC:37536
• Gene
  PIRC32
• Long gene name
  piwi-interacting RNA cluster 32
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313811
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32995
• Gene
  SNORD114-7
• Long gene name
  small nucleolar RNA, C/D box 114-7
• Synonyms
  • 14q(II-7)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767583
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003199.1
• Classification
  • Small nucleolar RNAs, C/D box

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products