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Long name
MCM4/CDC21 Primary Polyclonal Antibody
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Also known as
MCM4/CDC21 PAb
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
MCM4/CDC21
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Specificity
This is a highly specific antibody against MCM4/CDC21.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Polyclonal antibody
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Clone number
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MCM4
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Tested Applications
WB, IHC-P, IF(IHC-P)
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym names
CDC21; CDC21 homolog; CDC54; DNA replication licensing factor MCM 4; DNA replication licensing factor MCM4; hCdc 21; hCdc21; Homolog of S. pombe cell devision cycle 21; KIAA4003; mcdc21; MCM 4; MCM 4 minichromosome maintenance deficient 4; MCM4; MCM4 minichromosome maintenance deficient 4; MCM4 minichromosome maintenance deficient 4, mitotin S. cerevisiae; MCM4_HUMAN; Mcmd4; MGC33310; Minichromosome Maintenance 4; Minichromosome maintenance complex component 4; Minichromosome maintenance deficient S. cerevisiae 4; Minichromosome maintenance deficient 4; Minichromosome maintenance deficient 4 homolog S. cerevisiae; Minichromosome maintenance, S. cerevisiae, homolog of, 4; mKIAA4003; P1 CDC21; P1-CDC21; P1CDC21; 19G antibody AI325074; AU045576; CDC 21; CDC 54.
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
