NMNAT3, human recombinant
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Catalog number
7562-50
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Price
Please ask
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Size
50 ug
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Synonyms
Nicotinamide Mononucleotide Adenylyl-transferase 3; NMN Adenylyltransferase 3; NaMN Adenylyltransferase 3; EC 2.7.7.18; EC 2.7.7.1
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Alternative_names
Nicotinamide Mononucleotide Adenylyl-transferase 3; NMN Adenylyltransferase 3; NaMN Adenylyltransferase 3; EC 2.7.7.18; EC 2.7.7.1
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Description
Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
≥ 2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NAD+ per min.
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Results
≥ 2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1 µmol of NAD+ per min.
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Unit Definition
One unit is defined as the amount of enzyme that synthesizes 1 µmol of NAD+ per min.
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Molecular Weight
~27.0 kDa (monomer). Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
6 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
1 mg/ml in 50 mM sodium phosphate, pH 8.0 containing 300 mM sodium chloride, 1 mM DTT and 10% glycerol.
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Background Information
This is the Mitochondrial NMNAT isoform. It catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. It can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. It can use tiazofurin monophosphate as substrate. It can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. It fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. It protects against axonal degeneration following injury.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NMNAT3
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Short name
NMNAT3, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
NMNAT3, H. sapiens Rec.
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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