4-[4-[(5-Nitro-2-furanyl)methylene]-3,5-dioxo-1-pyrazolidinyl]benzoic acid ethyl ester
Cell-permeable. An irreversible ubiquitin-activating enzyme (E1) inhibitor (IC₅₀ < 10 μM). Blocks ubiquitylation and prevents ubiquitin-mediated proteasomal degradation. Inhibits NF-κB activation, blocks degradation of p53, increases p21 levels and induces apoptosis in vitro. Also causes an increase in sumoylation of proteins.
Is it cell-permeable?
≥98% by TLC
Is this a salt?
Protect from light and air
Maximum time for storage
DMSO (~25 mg/ml)
For cells, cell lines and tissues in culture till half confluency.