HAMSTER ANTI MOUSE CD81:FITC Antibody
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Catalog number
GEN215269
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Price
Please ask
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Size
0.025 miligrams
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Also known as
CD81
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Other names
CD81 antigen; CD81 antigen; CD81 antigen; 26 kDa cell surface protein TAPA-1; target of the antiproliferative antibody 1; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD81
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Gene name synonims
N/A
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Other gene names
Cd81; Cd81; Tapa1; Tapa-1; Tspan28; Tapa1
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
Eat2
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Host organism
Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD81 Recognises mouse and rat CD81, a 26kD cell surface glycoprotein that is also known as TAPA-1. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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Concentration
IgG concentration 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Flow Cytometry (FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD81-AS1, CD81
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Short name
ANTI MOUSE CD81:FITC Antibody
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Technique
anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
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Host
Hamster
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Label
FITC
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Species
Mouse, Hamsters, Mouses
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Alternative name
Anti-HAMSTER ANTI MOUSE CD81:FITC
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD81 molecule, CVID6 and S5.7 and TAPA1 and TSPAN28, CD81 and IDBG-22455 and ENSG00000110651 and 975, MHC class II protein complex binding, Cell surfaces, Cd81 and IDBG-212677 and ENSMUSG00000037706 and 12520, CD81 and IDBG-645214 and ENSBTAG00000047495 and 511435
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Gene info
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Identity
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Gene
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Long gene name
CD81 antisense RNA 1
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GenBank acession
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Locus
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Discovery year
2013-10-18
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Entrez gene record
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
CD81 molecule
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Synonyms gene
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Synonyms gene name
- CD81 antigen (target of antiproliferative antibody 1)
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Synonyms
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Locus
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Discovery year
1992-10-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tetraspanins
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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