2x SYBR Green I Hot-start Real-time PCR Ready Mix[2x SYBR Green I Hot-start Real-time PCR]

• Catalog number
  MBS433676
• Price
  Please ask
• Size
  NA

• Products_type
  Taq DNA Polymerase
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Hot start taq polymerases can be supplied as mix with dNTPs or Mg+ free Polymerases. The units are redifined for reactions but you can delute more this Hot start TAQ polymerase. Storage at -25 º C.
• Description
  Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.

• Gene target
  SYBR   Hot-start   [2x   SYBR   Hot-start  
• Short name
  2x SYBR I Hot-start Real-time PCR [2x SYBR I Hot-start Real-time PCR]
• Technique
  Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Label
  green
• Alternative name
  2x SYBR Green I Hot-start Real-time PCR test kit Ready Mix[2x SYBR Green I Hot-start Real-time PCR test kit]
• Alternative technique
  rtpcr, dna-amplification

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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