TIGAR-TAT, human recombinant

• Catalog number
  7218-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  TP53-induced glycolysis and apoptosis regulator (TIGAR)
• Alternative_names
  TP53-induced glycolysis and apoptosis regulator (TIGAR)
• Description
  Catalyzes the hydrolysis of fructose-2-6 bisphosphate
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Molecular Weight
  31.6 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 31.6 kDa protein containing 283 amino-acid residues, including the 269 residues of full-length TIGAR fused to a 14-residue C-terminal peptide containing the TAT transduction domain (GGGYGRKKRRQRRR).
• Amino acid sequence
  ARFALTVVRH GETRFNKEKI IQGQGVDEPL SETGFKQAAA AGIFLNNVKF THAFSSDLMR TKQTMHGILE RSKFCKDMTV KYDSRLRERK YGVVEGKALS ELRAMAKAAR EECPVFTPPG GETLDQVKMR GIDFFEFLCQ LILKEADQKE QFSQGSPSNC LETSLAEIFP LGKNHSSKVN SDSGIPGLAA SVLVVSHGAY MRSLFDYFLT DLKCSLPATL SRSELMSVTP NTGMSLFIIN FEEGREVKPT VQCICMNLQD HLNGLTETRG GGYGRKKRRQ RRR
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TIGAR-TAT  
• Gene symbol
  TAT, TIGAR, TAT-AS1, HTATSF1, HTATSF1P1, TRI-TAT2-2, TRI-TAT1-1, TRI-TAT3-1, TRI-TAT2-3, TRI-TAT2-1
• Short name
  TIGAR-TAT, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  TIGAR-TAT, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11573
• Gene
  TAT
• Long gene name
  tyrosine aminotransferase
• Locus
  16q22.2
• Discovery year
  2001-06-22
• Entrez gene record
  6898
• VEGA ID
  OTTHUMG00000137590

Gene info

• Identity
  HGNC:1185
• Gene
  TIGAR
• Long gene name
  TP53 induced glycolysis regulatory phosphatase
• Synonyms gene
  • C12orf5
• Synonyms gene name
  • chromosome 12 open reading frame 5
• Synonyms name
  • TP53-induced glycolysis and apoptosis regulator
• GenBank acession
  • AJ272206
• Locus
  12p13.32
• Discovery year
  2000-08-24
• Entrez gene record
  57103
• Pubmed identfication
  • 18945750
  • 19713938
  • 2442317
• RefSeq identity
  • NM_020375
• Classification
  • Sugar phosphatases
• VEGA ID
  OTTHUMG00000168124

Gene info

• Identity
  HGNC:51369
• Gene
  TAT-AS1
• Long gene name
  TAT antisense RNA 1
• GenBank acession
  • HY017421
  • BU568247
  • BM853033
• Locus
  16q22.2
• Discovery year
  2014-10-16
• Entrez gene record
  100132529
• RefSeq identity
  • NR_103851
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000176800

Gene info

• Identity
  HGNC:5276
• Gene
  HTATSF1
• Long gene name
  HIV-1 Tat specific factor 1
• Synonyms gene name
  • HIV TAT specific factor 1
• Synonyms
  • TAT-SF1
• GenBank acession
  • U76992
• Locus
  Xq26.3
• Discovery year
  2000-07-25
• Entrez gene record
  27336
• Pubmed identfication
  • 8849451
• RefSeq identity
  • NM_014500
• Classification
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000022510
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:13959
• Gene
  HTATSF1P1
• Long gene name
  HIV-1 Tat specific factor 1 pseudogene 1
• Synonyms gene
  • HTATSF1P
• Synonyms gene name
  • HIV TAT specific factor 1 pseudogene
  • HIV-1 Tat specific factor 1 pseudogene
• Synonyms
  • TAT-SF1-L
  • dJ1033B10.6
• Locus
  6p21.32
• Discovery year
  2003-11-26
• Entrez gene record
  387039
• VEGA ID
  OTTHUMG00000031101

Gene info

• Identity
  HGNC:34587
• Gene
  TRI-TAT2-2
• Long gene name
  tRNA-Ile (anticodon TAT) 2-2
• Synonyms gene
  • TRNAI2
• Synonyms gene name
  • transfer RNA isoleucine 2 (anticodon UAU)
  • transfer RNA-Ile (TAT) 2-2
• Synonyms
  • tRNA-Ile-TAT-2-2
• GenBank acession
  • HG983865
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189028
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34891
• Gene
  TRI-TAT1-1
• Long gene name
  tRNA-Ile (anticodon TAT) 1-1
• Synonyms gene
  • TRNAI18
• Synonyms gene name
  • transfer RNA isoleucine 18 (anticodon UAU)
  • transfer RNA-Ile (TAT) 1-1
• Synonyms
  • tRNA-Ile-TAT-1-1
• GenBank acession
  • HG983863
• Locus
  19q13.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189324
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34916
• Gene
  TRI-TAT3-1
• Long gene name
  tRNA-Ile (anticodon TAT) 3-1
• Synonyms gene
  • TRNAI21
• Synonyms gene name
  • transfer RNA isoleucine 21 (anticodon UAU)
  • transfer RNA-Ile (TAT) 3-1
• Synonyms
  • tRNA-Ile-TAT-3-1
• GenBank acession
  • HG983867
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189348
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34693
• Gene
  TRI-TAT2-3
• Long gene name
  tRNA-Ile (anticodon TAT) 2-3
• Synonyms gene
  • TRNAI6
• Synonyms gene name
  • transfer RNA isoleucine 6 (anticodon UAU)
  • transfer RNA-Ile (TAT) 2-3
• Synonyms
  • tRNA-Ile-TAT-2-3
• GenBank acession
  • HG983866
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189131
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34828
• Gene
  TRI-TAT2-1
• Long gene name
  tRNA-Ile (anticodon TAT) 2-1
• Synonyms gene
  • TRNAI13
• Synonyms gene name
  • transfer RNA isoleucine 13 (anticodon UAU)
  • transfer RNA-Ile (TAT) 2-1
• Synonyms
  • tRNA-Ile-TAT-2-1
• GenBank acession
  • HG983864
• Locus
  2p21
• Discovery year
  2008-08-29
• Entrez gene record
  100189265
• Classification
  • Cytoplasmic transfer RNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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