Human Recombinant UGDH

• Catalog number
  6368-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Human Recombinant UGDH
• Alternative_names
  UDP-glucose dehydrogenase, GDH, UDP-GlcDH, UDPGDH, UGD.
• Description
  Takes part in the biosynthesis of glycosaminoglycans
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: >0.1 unit/ml.
• Results
  >0.1 unit/ml
• Unit Definition
  One unit will oxidize 1.0 µmol of UDP-glucose to UDP-glucuronic acid per minute at pH 8.0 at 25°C.
• Molecular Weight
  59.5 kDa (533 aa, 1-494 aa + NT His-Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT, 0.1 M NaCl, and 1 mM EDTA.
• Background Information
  UGDH is part of the UDP-glucose/GDP-mannose dehydrogenase family and is a widely expressed enzyme localized in the liver. UGDH transfers UDP-glucose to UDP-glucuronate and thus takes part in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated products are ordinary molecules of the extracellular matrix and participate in signal transduction, cell migration, and cancer growth and metastasis.
• Amino acid sequence
  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UGDH  
• Gene symbol
  UGDH, UGDH-AS1
• Short name
  Recombinant UGDH
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. UGDH
• Alternative technique
  rec

Gene info

• Identity
  HGNC:12525
• Gene
  UGDH
• Long gene name
  UDP-glucose 6-dehydrogenase
• Synonyms gene name
  • UDP-glucose dehydrogenase
• GenBank acession
  • AF061016
• Locus
  4p14
• Discovery year
  1997-12-01
• Entrez gene record
  7358
• Pubmed identfication
  • 9737970
  • 10575217
• RefSeq identity
  • NM_003359
• VEGA ID
  OTTHUMG00000099371

Gene info

• Identity
  HGNC:40601
• Gene
  UGDH-AS1
• Long gene name
  UGDH antisense RNA 1
• Synonyms gene name
  • UGDH antisense RNA 1 (non-protein coding)
• Locus
  4p14
• Discovery year
  2011-11-07
• Entrez gene record
  100885776
• Pubmed identfication
  • 29901136
• RefSeq identity
  • NR_047679
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000160540

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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