GZMB Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

GZMB Lentiviral Vector CMV pLenti-GIII-CMV-RFP-2A-Puro
Gene symbol

TRIM27, IRX5, PLA2G3, IGHD2OR15-2A, EIF2A, CXCL2, RNVU1-2A, CHD3, PLPP1, TRAPPC2B
Short name

GZMB Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
Technique

Vectors
Label

puro
Species

Human, Humans
Alternative name

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-Red Fluorescent-2A-Puro)
Alternative to gene target

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), CCPI and CGL-1 and CGL1 and CSP-B and CSPB and CTLA1 and CTSGL1 and HLP and SECT, GZMB and IDBG-4054 and ENSG00000100453 and 3002, serine-type peptidase activity, nuclei, BT.19365 and IDBG-632083 and ENSBTAG00000010057 and 508646
Virus

cmv

Identity

HGNC:1918
Gene

CHD3
Long gene name

chromodomain helicase DNA binding protein 3
Synonyms
- Mi-2a
- ZFH
- Mi2-ALPHA
GenBank acession
- U08379
Locus

17p13.1
Discovery year

1998-03-20
Entrez gene record

1107
Pubmed identfication
- 9326634
- 7560064
RefSeq identity
- NM_001005273
Classification
- Small nucleolar RNA protein coding host genes
- NuRD complex subunits
- PHD finger proteins
VEGA ID

OTTHUMG00000150427

Identity

HGNC:10710
Gene

TRAPPC2B
Long gene name

trafficking protein particle complex subunit 2B
Synonyms gene
- SEDLP
- TRAPPC2P1
Synonyms gene name
- spondyloepiphyseal dysplasia, late, pseudogene
- trafficking protein particle complex 2 pseudogene 1
- trafficking protein particle complex 2B
Synonyms
- SEDLP1
- MIP-2A
Locus

19q13.43
Discovery year

2000-01-20
Entrez gene record

10597
Pubmed identfication
- 11134351
- 24098805
RefSeq identity
- NM_001355204
VEGA ID

OTTHUMG00000183279

Name

Transduction, Genetic
Concept

Scope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Tree numbers
- E05.393.350.800
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data