anti-5Methylcytosine antibody mouse IgM (clone 5MC-CD), Biotin-conjugated, Immuno Blot, IF/ICC, Azide and carrier free
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Catalog number
51-004
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Price
Please ask
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Size
50ug
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. AS ONE INTERNATIONAL supplies azide free antibodies on request. A carrier can hold protected the subject it is supporting for the period needed by its application as stated by AS ONE INTERNATIONAL.
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Properties
If you buy Antibodies supplied by AS ONE INTERNATIONAL they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Biotinylated
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
M6PR, MIA, NOD2, CTLA4
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Short name
anti-5Methylcytosine antibody mouse IgM (clone 5MC-CD), -conjugated, Immuno Blot, IF/ICC, free
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Technique
Antibody, anti-, Mouse, anti, Blot, IgM, IF, antibody to, antibodies, antibodies against human proteins, antibodies for, western Blots, antibody Conjugates, IgMs, mouses
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Host
mouse
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Isotype
IgM, IgM
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Label
Biotin
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Species
Mouses
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Alternative name
Antibody to5Methylcytosine (antibody to-) mouse Immunoglobulin M (clonality 5MC-CD), biotinilated-coupled, Immuno Blot, IF/ICC, Azide and carrier free
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Alternative technique
antibodies, murine, western, immunoglobulin-m
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Clone name
5MC-CD
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Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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