RAT ANTI MOUSE CD13:Biotin Antibody
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Catalog number
GEN215586
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Price
Please ask
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Size
0.025 miligrams
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Also known as
CD13
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Other names
aminopeptidase N; Aminopeptidase N; aminopeptidase N; aminopeptidase M; alanyl aminopeptidase; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase; alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Membrane protein p161; Microsomal aminopeptidase
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD13
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Gene name synonims
N/A
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Other gene names
Anpep; Anpep; Apn; AP-M; AP-N; Cd13; P150; Lap-1; Lap1; AP-N; mAPN; AP-M
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
R3-63
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Host organism
Rat (Rattus norvegicus)
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD13 This item recognises mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Clone R3-63 has been reported to block mouse APN enzyme activity; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Biotin (Purified IgG conjugated to Biotin - liquid)
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Concentration
IgG concentration 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Flow Cytometry (FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translation
anticorps
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Gene target
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Gene symbol
ANPEP
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Short name
ANTI MOUSE CD13: Antibody
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Technique
anti mouse, Antibody, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
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Host
Rat
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Label
Biotin
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Species
Mouse, Mouses, Rats
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Alternative name
Anti-RAT ANTI MOUSE CD13:Biotin
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Alternative technique
antibodies, murine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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