-
Target antigen
IL4
-
Clonality
Polyclonal antibody
-
Clone
Polyclonal antibody
-
Raised in
rabbit
-
Type of the antibody
IgG polyclonal antibody
-
Product form
freeze-dried
-
Reacts with species
human, rat Predicted to work with: mouse
-
Analyses
WB,IHC-P
-
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IL-4(72-88aa, AATVLRQFYSHHEKDTR).
-
Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
-
Purification
Immunogen affinity purified.
-
Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
-
Storage condtions
Keep the IL4 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
-
Tips
The IL4 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
-
Background
Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
-
Related articles
1. Prokopchuk, O.; Liu, Y.; Henne-Bruns, D.; Kornmann, M. Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions. Br J Cancer.2005 Mar 14;92(5):921-8. 2. Arai, N.; Nomura, D.; Villaret, D.; DeWaal Malefijt, R.; Seiki, M.; Yoshida, M.; Minoshima, S.; Fukuyama, R.; Maekawa, M.; Kudoh, J.; Shimizu, N.; Yokota, K.; Abe, E.; Yokota, T.; Takebe, Y.; Arai, K. Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J. Immun. 142: 274-282, 1989.
-
Gene Name
IL4
-
Protein Name
Interleukin-4(IL-4)
-
Gene Full Name
interleukin 4
-
Synonyms
B cell growth factor 1 antibody|B cell IgG differentiation factor antibody|B Cell Stimulatory Factor 1 antibody|B-cell stimulatory factor 1 antibody|BCGF 1 antibody|BCGF1 antibody|Binetrakin antibody|BSF 1 antibody|BSF-1 antibody|BSF1 antibody|HCGF antibody|Hodgkin's Cell Growth Factor antibody|IA Inducing Factor antibody|IGG1 induction factor antibody|IL 4 antibody|IL-4 antibody|IL4 antibody|IL4_HUMAN antibody|Il4e12 antibody|Interleukin 4 antibody|Interleukin 4, isoform 1 antibody|Interleukin-4 antibody|Interleukin4 antibody|Lymphocyte stimulatory factor 1 antibody|Macrophage fusion factor antibody|Mast cell growth factor 2 antibody|MCGF2 antibody|MFF antibody|MGC79402 antibody|Pitrakinra antibody|T cell growth factor 2 antibody|TCGF2 antibody
-
Uniprot ID
P05112
-
Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Gene
Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is used for dendritic and T cell therapy. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13. Recombinant, GMP rec. E. coli interleukin-4 for cell culture supplied by GENTAUR. Free samples on request.
-
French translation
anticorps
