Acetyl Lysine (Biotin) Antibody
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Catalog number
6125-50
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Price
Please ask
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Size
50 μg
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Antibody Target
Acetyl Lysine
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Target Alternative Name
Acetyl Lysine
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Antibody Type
Polyclonal
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Species Reactivities
Multiple species
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Immunogen Sequence
Acetylated KLH
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
50 µg of antibody in 200 µl PBS, containing 0.01% sodium azide.
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Purification
Affinity purified
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Application
Western blot, Immunoprecipitation, ELISA, Immunofluorescence
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Application And Usages
Western blot, IP, ELISA and IF.
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Description
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of lysine involves acetylation. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Protein post-translational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization. The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drμgs currently in development.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Verified
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ELISA Verified
Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
N terminal acetylation or CH3CO as epigenetic regulation of Lysine ( ) Antibody by NATs.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Gene symbol
PLOD2, KMT5A, KDM6B
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Short name
Lysine ( ) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Label
Biotin
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Alternative name
Acetyl Lysine (biotinilated) (antibody to-)
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Alternative technique
antibodies
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Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
lysine demethylase 6B
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Synonyms gene
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Synonyms gene name
- jumonji domain containing 3
- jumonji domain containing 3, histone lysine demethylase
- lysine (K)-specific demethylase 6B
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Synonyms
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GenBank acession
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Locus
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Discovery year
2004-02-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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