Mouse Anti-Rat SNAT1 Monoclonal IgG1, Clone S104-32
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Catalog numberSMC-401D-A655
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PricePlease ask
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Size100 µg
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CloneS104-32
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ImmunogenFusion protein amino acids 1- 63 of rat SNAT1
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Antibody s full descriptionMouse Anti-Rat SLC38A1 Monoclonal IgG1 Antibody, Clone: S104-32: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameSLC38A1 Antibody, amino acids transporter A1 Antibody, ATA1 Antibody, NAT2 Antibody, sodium coupled neutral amino acids transporter 1 Antibody, Amino acid transporter system A1 Antibody, N-system amino acid transporter 2 Antibody, S38A1 Antibody, SAT1 Antibody, SNAT 1 Antibody, Solute carrier family 38 member 1 Antibody, System A amino acid transporter 1 Antibody, System N amino acid transporter 1 Antibody
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Raised inMouse
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Antibody s targetSLC38A1
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Primary research fieldsNeuroscience, Pumps/Transporters, Neurotransmitter Transporters, Cell Signaling, Trafficking
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~50kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-401 was sufficient for detection of SNAT1 in 20 µg of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe sodium-coupled neutral amino acid transporters (SNAT) of the SLC38 gene family include System A subtypes SNAT1, SNAT2 and SNAT4 and System N subtypes SNAT3 and SNAT5. The SLC38 transporters are essential for the uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. The SNAT1 protein, also designated ATA1 or NAT2 is encoded by the human gene SLC38A1 which maps to chromosome 12q13.11. SNAT1 is responsible for the transport of glutamine, an intermediate in the synthesis of urea, and may be involved in the generation of glutamate in the retina. SNAT1 protein may be detected in some tissues such as heart, brain and placenta and expression levels are enriched in certain neuronal populations within the CNS. SNAT1 is not present in astrocytes.
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Bibliography1. Hatanaka T., et al. ( 2000) Biochim. Biophys. Acta 1467: 1-6. 2. Wang H., et al. (2000) Biochem. Biophys. Res. Commun. 273: 1175-1179. 3. Gu S., et al. (2001) J. Biol. Chem. 276: 24137-24144. 4. Freeman T.L., et al. (2002) Arch. Biochem. Biophys. 400: 215-222. 5. Palii S.S., et al. (2004) J. Biol. Chem. 279: 3463-3471. 6. Sidoryk M., et al. (2004) Neuroreport. 15: 575-578. Erratum in: 2004. Neuroreport. 15: 533. 7. Desforges,M., et al. (2006) Am. J. Physiol. Cell. Physiol. 290: 305-312.
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Released date16-May-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_620187.1
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Gene number170567
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Protein numberQ9JM15
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (SMC-401). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (SMC-401) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SNAT1 Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (SMC-401). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (SMC-401) at 1:1000. Mouse Anti-SNAT1 Antibody [S104-32] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-401) Mouse Anti-SLC38A1 Antibody [S104-32] used in Western Blot (WB) on Rat brain membrane lysate (SMC-401)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolIGKV1D-32, SNORD115-32, PPP1R1B, IGLV3-32, IGHV3-32, IGKV1-32, CUTC, ZNF138, LINC01234, LUARIS
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Short nameMouse Anti- SNAT1 Monoclonal IgG1, Clone S104-32
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 655
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat SNAT1 monoclonal IgG1, clonality S104-32
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Alternative techniquemurine, antibodies
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Clone nameS104-32
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-32
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-32
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory inhibitor subunit 1B
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 1B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-32 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 3-32
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-32 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-32
- immunoglobulin heavy variable 3-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1-32
- immunoglobulin kappa variable 1-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecutC copper transporter
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Synonyms gene name
- cutC copper transporter homolog (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 138
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Synonyms gene name
- zinc finger protein 138 (clone pHZ-32)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-02-11
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Entrez gene record
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1234
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Synonyms
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Locus
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Discovery year2014-02-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelncRNA upregulator of antiviral response interferon signaling
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Synonyms
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Synonyms name
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Locus
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Discovery year2017-03-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
