AGO2 Polyclonal Antibody

• Catalog number
  CAC07145
• Price
  Please ask
• Size
  50 μg

• Other name
  Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2,PAZ Piwi domain protein,Protein slicer,EIF2C2,AGO2
• Immunogen
  Recombinant human Protein argonaute-2 protein (517-818AA)
• Verified reactivity
  Human, Mouse
• Verified applications
  ELISA,WB,IHC,ChIP
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Protein number
  Q9UKV8
• Gene number
  Please refer to GenBank
• NCBI number
  Please refer to NCBI
• Purity
  Caprylic Acid Ammonium Sulfate Precipitation Purified
• Storage recommendation
  Aliquot and store at -20°C. Minimize freezing and thawing.
• Use before
  1 year
• Shipping requirements
  Blue ice
• Estimated production time
  3-7 business days
• Supplementary information
  Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
• Notes
  For research use only. Not for diagnostic procedures.
• Hyperlink
• Properties
  If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  AGO2  
• Gene symbol
  AGO2
• Short name
  AGO2 Polyclonal Antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Label
  Unconjugated
• Alternative name
  AGO2 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:3263
• Gene
  AGO2
• Long gene name
  argonaute RISC catalytic component 2
• Synonyms gene
  • EIF2C2
  • CASC7
• Synonyms gene name
  • eukaryotic translation initiation factor 2C, 2
  • cancer susceptibility candidate 7 (non-protein coding)
• Synonyms
  • hAGO2
  • Q10
  • LINC00980
• Synonyms name
  • argonaute 2
• GenBank acession
  • AF121255
• Locus
  8q24.3
• Discovery year
  2000-01-14
• Entrez gene record
  27161
• Pubmed identfication
  • 10534406
  • 12906857
• RefSeq identity
  • NM_012154
• Classification
  • Argonaute RISC component family
  • RISC loading complex
  • Endoribonucleases
• VEGA ID
  OTTHUMG00000164232

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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