FGF-basic 147, human recombinant

• Catalog number
  4036-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  FGFB
• Alternative_names
  FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
• Description
  A growth factor involved in angiogenesis, wound healing and embryonic development
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Biological activity
  EC50 is typically 0.67-1.45 ng/ml. The activity was determined by the dose-dependent stimulation of mouse BALB/c 3T3 cells.
• Results
  0.67-1.45 ng/ml
• Molecular Weight
  16.5 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized from a 10 mM Na2PO4, pH 8.0.
• Reconstitution Instructions
  Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Background Information
  Fibroblast Growth Factors, FGFs, are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154) is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, and having a molecular mass of 16.5 kDa.
• Amino acid sequence
  MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  FGF   147  
• Gene symbol
  FGF18, FGF17, NUDT6, FGF4, FGF1
• Short name
  FGF- 147, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  FGF-basic 147, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:3674
• Gene
  FGF18
• Long gene name
  fibroblast growth factor 18
• Synonyms
  • FGF-18
  • ZFGF5
• GenBank acession
  • AB007422
• Locus
  5q35.1
• Discovery year
  1998-12-22
• Entrez gene record
  8817
• Pubmed identfication
  • 9660775
  • 9742123
• RefSeq identity
  • NM_033649
  • NM_003862
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000130464

Gene info

• Identity
  HGNC:3673
• Gene
  FGF17
• Long gene name
  fibroblast growth factor 17
• Synonyms
  • FGF-13
• GenBank acession
  • AB009249
• Locus
  8p21.3
• Discovery year
  1998-12-22
• Entrez gene record
  8822
• Pubmed identfication
  • 9514906
  • 9751161
• RefSeq identity
  • NM_003867
• Classification
  • Fibroblast growth factor family
• VEGA ID
  OTTHUMG00000097051

Gene info

• Identity
  HGNC:8053
• Gene
  NUDT6
• Long gene name
  nudix hydrolase 6
• Synonyms gene name
  • nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Synonyms
  • gfg-1
  • gfg
  • FGF2AS
  • FGF-AS
• GenBank acession
  • AF019632
• Locus
  4q28.1
• Discovery year
  1999-09-03
• Entrez gene record
  11162
• Pubmed identfication
  • 7984147
  • 10022609
• RefSeq identity
  • NM_007083
• Classification
  • Nudix hydrolase family
• VEGA ID
  OTTHUMG00000039507

Gene info

• Identity
  HGNC:3682
• Gene
  FGF4
• Long gene name
  fibroblast growth factor 4
• Synonyms gene
  • HSTF1
• Synonyms gene name
  • heparin secretory transforming protein 1
• Synonyms
  • K-FGF
  • HBGF-4
  • HST
  • HST-1
  • KFGF
• Synonyms name
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • transforming protein KS3
• GenBank acession
  • M17446
• Locus
  11q13.3
• Discovery year
  1988-04-20
• Entrez gene record
  2249
• Pubmed identfication
  • 1611909
• RefSeq identity
  • NM_002007
• Classification
  • Fibroblast growth factor family
  • Receptor ligands
• VEGA ID
  OTTHUMG00000167887

Gene info

• Identity
  HGNC:3665
• Gene
  FGF1
• Long gene name
  fibroblast growth factor 1
• Synonyms gene
  • FGFA
• Synonyms gene name
  • fibroblast growth factor 1 (acidic)
• Synonyms
  • AFGF
  • ECGF
  • ECGFA
  • ECGFB
  • HBGF1
  • ECGF-beta
  • FGF-alpha
  • GLIO703
• Synonyms name
  • heparin-binding growth factor 1
  • endothelial cell growth factor, alpha
  • endothelial cell growth factor, beta
• GenBank acession
  • X59065
• Locus
  5q31.3
• Discovery year
  1986-01-01
• Entrez gene record
  2246
• Pubmed identfication
  • 1697263
  • 3523756
• RefSeq identity
  • NM_000800
• Classification
  • Fibroblast growth factor family
  • Receptor ligands
• VEGA ID
  OTTHUMG00000059703

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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