Glyoxalase I Activity Assay Kit (Colorimetric)

• Catalog number
  K591-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit in the market to detect Glyoxalase I activity
• Summary
  • Detection method- Absorbance (240 nm) • Sample type- tissue and cell culture lysate, • Species reactivity- Mammalian • Application- Measurement of Glyoxalase I as low as 0.05 mU
• Detection Method
  Colorimetric (OD 240 nm)
• Species Reactivity
  Mammalian
• Applications
  Measurmente of Glyoxalase I activity in biological samples
• Sample Type
  Cell and tissue lysates,purified enzyme
• Features Benefits
  • Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Glyoxalase I
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glyoxalase I (GloI, lactoylglutathione lyase, EC 4.4.1.5) is a cytosolic enzyme that participates in the Glyoxalase system. The glyoxalase system is a metabolic pathway that detoxifies α-ketoaldehydes such as methylglyoxal to D-lactic acid, via the intermediate S-D-lactoyl-glutathione (SLG). Specifically, GloI catalyzes the formation of SLG from methylglyoxal and reduced Glutathione. It is overexpressed in several human cancers such as colon, breast, prostate and melanoma. GloI also enhances resistance to antitumor agent-induced apoptosis in human leukemia cells. In addition, recent findings have linked GloI to numerous anxiety-like behaviors including psychiatric diseases (depression, schizophrenia and autism) and pain. BioVision’s Glyoxalase I Activity Assay Kit utilizes the ability of an active GloI to catalyze the formation of SLG by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm. Our assay kit is simple, sensitive and can detect as low as 0.05 mU of Glo I activity in biological samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Glyoxalase   Activity   Kit  
• Short name
  Glyoxalase I Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Glyoxalase I Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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