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Red Fluorescent Protein

Red Fluorescent Protein is available 3 times from Biovision labs

3994-100 | Red Fluorescent Protein (dsRed) Antibody size: 100 µg | 407.76 USD

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6005-1 | Red Fluorescent Protein - R-PE (R-Phycoerythrin) size: 1 mg | 240.29 USD

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6005-5 | Red Fluorescent Protein - R-PE (R-Phycoerythrin) size: 5 mg | 715.51 USD

Catalog number 6005-5
Supplier Biovision
Price 715.51 USD
Size 5 mg
Description Fluorescent protein which can be cross linked or conjugated to other molecules to make fluorescent probes.
Shelf Life 60 months
Appearance Suspension
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Additional description Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
Conjugation red dye
Gene targetRed Protein R-PE R-Phycoerythrin
Short name Red Protein - R-PE (R-Phycoerythrin)
Alternative name Red Fluorescent Protein - R-peroxidase (R-Phycoerythrin)
1. Gene info
Identity 3492
Gene ETV3
Long gene name ETS variant transcription factor 3
Synonyms gene name
  • ets variant gene 3, ETS family transcriptional repressor
  • ETS variant 3
Synonyms
  • PE-1
GenBank acession
  • BC022868
Locus 1q23.1
Discovery year 1994-12-15
Entrez gene record 2117
Pubmed identfication
  • 8020980
RefSeq identity
  • NM_005240
Classification
  • ETS transcription factor family
Havana BLAST/BLAT OTTHUMG00000034298
2. Gene info
Identity 10293
Gene RPE
Long gene name ribulose-5-phosphate-3-epimerase
Locus 2q34
Discovery year 2001-06-22
Entrez gene record 6120
RefSeq identity
  • NM_006916
Havana BLAST/BLAT OTTHUMG00000154690
Product images
    #0#.jpegRed Fluorescent Protein - R-PE (R-Phycoerythrin)
Synonyms RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Alternative_names RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Recombinant No
Source Febico’s proprietary red algae
Purity by SDS-PAGE A566/A280 ≥ 5.5
Assay Absorbance
Purity A566/A498 ≤1.5
Molecular Weight 240 kDa
Storage Temp. 2-8°C
Shipping Gel pack
Physical form description R-PE is supplied in 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.
Reconstitution Instructions Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tubes) or desalted in columns (BioVision Cat # 6564-25) prior to use, to remove any remaining ammonium sulphate.
Background Information R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.
Antibody Target RFP (dsRed)
Target Alternative Name Red Fluorescent Protein
Antibody Type Monoclonal
Clone# BV3-2
Species Reactivities All species
Immunogen Sequence Recombinant Red Fluorescent Protein (dsRed) expressed from E. coli (Cat.# 4997-100)
Accession # P00273
Gene Id 3229046
Form Liquid
Formulation in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
Purification Affinity purified
Application Western blot, Immunofluorescence, Immunohistochemistry
Application And Usages The antibody can be used for Western blotting (1-4 μg/ml), immunofluorescence (10-20 μg/ml) and immunohistochemistry (10-20 μg/ml). However, the optimal conditions should be determined individually.
Storage Condition -20 °C
Shipping Condition gel pack
Western Blot Verified Verified
Immunocytochemistry Verified Verified
Immunofluorescence Verified Verified
Immunoprecipitation Verified Not Verified
FACS Verified Not Verified
ELISA Verified Not Verified
ChIP Verified Not Verified
Flow Cytometry Verified Not Verified
Technique Antibody
Isotype Mouse IgG2b
Label Red
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Red Protein - R-PE (R-Phycoerythrin) | Technique alternative
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