Synonyms
RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Alternative_names
RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Recombinant
No
Source
Febico’s proprietary red algae
Purity by SDS-PAGE
A566/A280 ≥ 5.5
Assay
Absorbance
Purity
A566/A498 ≤1.5
Molecular Weight
240 kDa
Storage Temp.
2-8°C
Shipping
Gel pack
Physical form description
R-PE is supplied in 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.
Reconstitution Instructions
Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tubes) or desalted in columns (BioVision Cat # 6564-25) prior to use, to remove any remaining ammonium sulphate.
Background Information
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.
Antibody Target
RFP (dsRed)
Target Alternative Name
Red Fluorescent Protein
Antibody Type
Monoclonal
Clone#
BV3-2
Species Reactivities
All species
Immunogen Sequence
Recombinant Red Fluorescent Protein (dsRed) expressed from E. coli (Cat.# 4997-100)
Accession #
P00273
Gene Id
3229046
Form
Liquid
Formulation
in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
Purification
Affinity purified
Application
Western blot, Immunofluorescence, Immunohistochemistry
Application And Usages
The antibody can be used for Western blotting (1-4 μg/ml), immunofluorescence (10-20 μg/ml) and immunohistochemistry (10-20 μg/ml). However, the optimal conditions should be determined individually.
Storage Condition
-20 °C
Shipping Condition
gel pack
Western Blot Verified
Verified
Immunocytochemistry Verified
Verified
Immunofluorescence Verified
Verified
Immunoprecipitation Verified
Not Verified
FACS Verified
Not Verified
ELISA Verified
Not Verified
ChIP Verified
Not Verified
Flow Cytometry Verified
Not Verified
