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Products_type
Active Protein
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Products_short_name
[Eck, N-terminal His tag]
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Other_names
[ephrin type-A receptor 2; Ephrin type-A receptor 2; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase; EPH receptor A2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK]
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Other_gene_names
[EPHA2; EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6; ECK]
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Reactivity
Human
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Purity
80% by SDS-PAGE
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Form
25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.
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Storage_stability
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
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Description
For cells, cell lines and tissues in culture till half confluency. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Eck, Recombinant, , N-terminal His tag (EphA2, Mpk-5, Native Epithelial Kinase, Eph receptor tyrosine kinase A2)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Eck, Rec., H. sapiens, N-terminal histidine detection labelled (EPH receptor A2, Mpk-5, Native Epithelial cellular phosphorylation catalyst, Eph receptor tyrosine phosphorylation catalyst A2)
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Alternative technique
rec
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Alternative to gene target
EPH receptor A2, ARCC2 and CTPA and CTPP1 and CTRCT6 and ECK, EPHA2 and IDBG-91407 and ENSG00000142627 and 1969, transferase activity, Cell surfaces, Epha2 and IDBG-202005 and ENSMUSG00000006445 and 13836, EPHA2 and IDBG-645870 and ENSBTAG00000000815 and 512798
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Tissue
cell
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