Superoxide Dismutase (SOD), human recombinant

• Catalog number
  4802-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Superoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA
• Alternative_names
  Superoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA
• Description
  Enzyme that catalyze the dismutation of superoxide
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  BioVision recombinant Superoxide Dismutase is fully biologically active when compared to standard. Activity tests were carried using SOD activity assay kit (K335-100). The activity assay kit showed that the calculated activity was ~40,000 U/mg. This was calculated from the amount (µg) required to inhibit the Xanthine Oxidase activity by 50%
• Unit Definition
  One unit is defined as the amount of enzyme that will cause a 50% reduction of the rate of WST-1 formazan formation
• Molecular Weight
  16.8 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized powder
• Reconstitution Instructions
  Reconstitute in H₂O to a concentration >100 ug/ml. The solution can then be diluted into other aqueous buffers
• Background Information
  Superoxide Dismutase (SOD) is an oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a total molecular mass of 31,608 Dalton. The Cu/Zn SOD is purified by proprietary chromatographic techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Superoxide   Dismutase   SOD  
• Gene symbol
  SOD3
• Short name
  Superoxide Dismutase (SOD), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Superoxide Dismutase (antioxidant enzyme), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11181
• Gene
  SOD3
• Long gene name
  superoxide dismutase 3
• Synonyms gene name
  • superoxide dismutase 3, extracellular
• Synonyms
  • EC-SOD
• Locus
  4p15.2
• Discovery year
  1988-05-08
• Entrez gene record
  6649
• Classification
  • Superoxide dismutases
• VEGA ID
  OTTHUMG00000128565

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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