LEF-1 (Phospho-Ser42) Antibody

Applications

WB ELISA
Source

Rabbit
Reactivity

H, M, R
MW kDa

44 kDa
Target Modification

Phospho
Modification Sites

H:S42 M:S40 R:S40
Immunogen

The antiserum was produced against synthesized peptide derived from human LEF-1 around the phosphorylation site of Ser42.
Immunogen Range

8-57
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

LEF-1 (Phospho-Ser42) Antibody detects endogenous levels of LEF-1 only when phosphorylated at Ser42.
Gene ID

51176
Swiss Prot No

Q9UJU2
OMIM No

153245
Unigene No

Hs.555947
NCBI Gene Symbol

LEF1
Synonyms

LEF1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
Storage Stability

Stable at -20°C for at least 1 year.
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality

Polyclonal
Concentration

1 mg/ml
Properties

If you buy Antibodies supplied by Assay Biotech they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Gene target

LEF-1 Phospho-Ser42
Gene symbol

LEF1-AS1, LEF1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
Short name

LEF-1 (Phospho-Ser42) Antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Alternative name

LEF-1 (phosphorilated-Ser42) (antibody to-)
Alternative technique

antibodies

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data