Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
Antibody Concentration: 0.1 miligram per 1 mililiter
Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus,Rattus norvegicus
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Anti- RT1.D (Clone OX-17) (mouse IgG1)
Anti-Anti-Rat RT1.D Biotin (Clone OX-17) (mouse IgG1)
Long gene name DiGeorge syndrome critical region gene 11
Synonyms gene name
- DiGeorge syndrome critical region gene 11
- DiGeorge syndrome critical region gene 11 (non-protein coding)
Discovery year 2002-11-13
Entrez gene record 25786
- Long non-coding RNAs with non-systematic symbols
Havana BLAST/BLAT OTTHUMG00000186100
Long gene name piwi-interacting RNA cluster 91
Discovery year 2009-11-05
Entrez gene record 100313900
- Piwi-interacting RNA clusters